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Clinical Chemistry 21: 1474-1478, 1975;
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Clinical Chemistry, Vol 21, 1474-1478, Copyright © 1975 by the American Association for Clinical Chemistry

Rapid, Specific Assay for Plasma Cortisol by Competitive Protein Binding

Paul M. Keane 1, Judith Stuart 1, Jean Mendez 1, Sandra Barbadoro 1, and William H. C. Walker 2

1 Department of Clinical Chemistry, St. Joseph's Hospital, Hamilton, Ontario, L8N 1Y4, Canada.
2 Department of Clinical Chemistry, McMaster University Medical Centre, 1200 Main St. West, Hamilton, Ontario, L8S 4J9, Canada.

We describe a modified competitive protein-binding method for assay of plasma cortisol. Plasma samples are deproteinized by dilution with an ethanol/phosphate buffer, followed by heating at 100 °C for 2 min. Horse serum is used as the source of transcortin. Free radioactivity is separated from the protein-bound component by partition into liquid scintillation counting fluid. A batch of 30 tubes can be ready for counting within 60 min. The assay has better specificity and precision than a competitive protein-binding assay in which ethanol extraction and Florisil adsorbent are used, and results correlate well with those of a specific radioimmunoassay method.

Submitted on April 25, 1975
Accepted on June 20, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.