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Clinical Chemistry 21: 1486-1489, 1975;
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Clinical Chemistry, Vol 21, 1486-1489, Copyright © 1975 by the American Association for Clinical Chemistry

Gas Chromatographic/Thin-Layer Chromatographic Analysis of Acetylated Codeine and Morphine in Urine

Naresh C. Jain 1, Thomas C. Sneath 2, Robert D. Budd 2, and Wai J. Leung 2

1 The Departments of Pharmacology and of Community Medicine and Public Health, University of Southern California School of Medicine, Los Angeles, Calif. 90033.Rancho Los Amigos Hospital, 7601 E. Imperial Highway, Downey, Calif. 90242.
2 Rancho Los Amigos Hospital, 7601 E. Imperial Highway, Downey, Calif. 90242.

This procedure positively identifies codeine and morphine in urine. Urine samples are hydrolyzed and extracted with organic solvent, and the extracts are evaporated and acetylated. The presence of codeine and morphine is ascertained by gas chromatography (3% OV-25 and 3% Poly-A 103 columns) and confirmed by thin-layer chromatography (system: ethyl acetate/acetone/concd ammonium hydroxide, 100/10/4.5 by vol; reagent: iodoplatinate). As little as 0.5 mg each of codeine and morphine per liter, in free and conjugated forms, is detectable by this method.

Submitted on February 10, 1975
Accepted on June 23, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.