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Clinical Chemistry 21: 1490-1492, 1975;
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Clinical Chemistry, Vol 21, 1490-1492, Copyright © 1975 by the American Association for Clinical Chemistry

Rapid Estimation of Hemoglobin A2 without Chromatography or Electrophoresis

Leo G. Morin 1

1 Veterans Administration Hospital, Laboratory Service; and the Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Decatur, Ga. 30033.

Hemoglobin A2 is batch fractionated with diethylaminoethyl Bio-Gel A (Bio-Rad Laboratories) equilibrated with a tris(hydroxymethyl)aminomethane HCl buffer (pH 7.68, 8.75 mmol/liter, 6.36 mmol of Cl- per liter). Hemoglobin A1 and F become bound to the resin, allowing the separation and quantitation of A2. Hemoglobin S alters the equilibrium condition, but adjustments are easily made so that A2 can be separated in the presence of S. The procedure is simpler than electrophoretic or chromatographic methods, requires 5 min, is accurate (A2 fraction is at least 94% A2, less than 6% A1), precise (SD ±0.24 for duplicates), and has a normal limit of 2.6 ± 1.02%.


Key Words: beta-thalassemia • diethylaminoethyl gel • normal (reference) values

Submitted on May 11, 1975
Accepted on June 23, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.