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Clinical Chemistry 21: 1582-1584, 1975;
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Clinical Chemistry, Vol 21, 1582-1584, Copyright © 1975 by the American Association for Clinical Chemistry

Measurement of Spectral Bandwidth, as Exemplified with the Beckman "Enzyme Analyzer System TR Spectrophotometer"

Richard B. Passey 1, Ronald L. Gillum 1, and John B. Fuller 1

1 Clinical Laboratories, University of Texas Medical Branch, Galveston, Tex. 77550

This paper describes simple methods for determining spectral bandwidth by measurement and by calculation. We measured the spectral bandwidth of the monochromator from the Beckman Enzyme Analyzer TR, and found it to be 3.5-4.0 nm (av 3.8) by two different methods. We visually estimated it to be 4 nm. Using the specifications for the diffraction grating, focal length, and fixed slit width, we calculated the theoretical spectral bandwidth to be 3.6 nm.

Submitted on May 30, 1975
Accepted on July 7, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.