Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 21: 1630-1631, 1975;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Beher, W. T.
Right arrow Articles by Lin, G. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Beher, W. T.
Right arrow Articles by Lin, G. J.

Clinical Chemistry, Vol 21, 1630-1631, Copyright © 1975 by the American Association for Clinical Chemistry

Specificity of Oxidation of Bile-Salt Hydroxyl Groups by Crude Extracts of Pseudomonas Testosteroni (ATCC 11996) Used in Determining Bile Salts

William T. Beher 1 and Grace J. Lin 1

1 Edsel B. Ford Institute for Medical Research, Henry Ford Hospital, Detroit, Mich. 48202

Recent experimental evidence suggests that, presumably as a result of mutation, crude extracts of Pseudomonas testosteroni (ATCC 11996) now contain amounts of 7agr-and 12agr-hydroxysteroid dehydrogenate activity that invalidate the results of bile-salt determinations. To confirm or deny this, we studied the specificity of hydroxysteroid dehydrogenase contained in crude extracts of currently available samples of this bacterium in the oxidation of bile-salt hydroxyl groups. The dehydrogenases in these extracts specifically oxidized 3agr-hydroxyl groups of cholate, chenodeoxycholate, and deoxycholate anions. No significant amount of 12agr-or 7agr-hydroxysteroid dehydrogenase activity was detected. Currently available crude extracts of Pseudomonas testosteroni (ATCC 11996) are therefore suitable for bile-salt quantification

Submitted on June 16, 1975
Accepted on July 24, 1975






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1975 by the American Association for Clinical Chemistry.