Clinical Chemistry
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Clinical Chemistry 21: 1632-1637, 1975;
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Clinical Chemistry, Vol 21, 1632-1637, Copyright © 1975 by the American Association for Clinical Chemistry

Simple, Sensitive Method for Measuring Plasmin and Plasminogen Activity in Plasma

Tetsuo Uete 1, Noriko Shimano 1, and Mamiko Morikawa 1

1 Kitano Hospital, Tazuke Kofukai Medical Research Institute, 3, Nishiogimachi, Kita-ku, Osaka City, Japan 530

We describe a sensitive, simple, and rapid method for measuring plasmin and plasminogen in plasma, with fibrinogen as substrate. The assay can be done within 1 h. Plasma is diluted 20-fold with buffer and 0.1 ml (5 µl of original plasma) is incubated for 5 min at 37 °C, with or without 400 units of streptokinase. Then 2.0 ml of fibrinogen solution is added, the mixture again incubated (37 °C, 5 min), and the reaction stopped. The amount of tyrosine liberated from the fibrinogen is measured and is related to activity. This method is suitable for routine clinical work if the same batch of fibrinogen is used


Key Words: substrate specificity • normal (reference) values • activation with streptokinase • fibrinogen as substrate • intermethod comparison

Submitted on March 24, 1975
Accepted on July 23, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.