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Clinical Chemistry 21: 1716-1720, 1975;
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Clinical Chemistry, Vol 21, 1716-1720, Copyright © 1975 by the American Association for Clinical Chemistry

Colorimetric Measurement of Ornithine Carbamoyl Transferase Activity in Plasma, and Results for a Supposedly Healthy Population

Alain Bagrel 1, Gérard Museur 1, and Gérard Siest 1

1 Centre de Médecine Préventive (Dir. Pr. Raoul Senault), 2, Avenue du Doyen Jacques Parisot, 54500 Vandoeuvre-les-Nancy, France.

Determination of ornithine carbamoyl transferase (EC 2.1.3.3) activity in plasma is important for detection of liver diseases. The assay established in this paper has been made optimum. A blank is needed containing both substrates, carbamoyl phosphate and ornithine. We used a new colorimetric assay, based on a complex with a phosphoferric-antipyrine reagent and diacetyl monoxime, to measure the citrulline formed. The highly sensitive assay permits low activities to be determined accurately. Values for blood plasma from 425 supposedly healthy people, varied from 0 to 16 U/liter (95th percentile), and 27% of this population showed an activity of less than 2 µmol of citrulline formed per minute per liter, 2 U/liter being the limit of the method’s sensitivity.


Key Words: reference values • enzyme activity • liver disease

Submitted on May 27, 1975
Accepted on August 6, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.