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Clinical Chemistry 21: 1735-1746, 1975;
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Clinical Chemistry, Vol 21, 1735-1746, Copyright © 1975 by the American Association for Clinical Chemistry

Evaluation of Kinetic Light Scattering as an Approach to the Measurement of Specific Proteins with the Centrifugal Analyzer. II. Theoretical Considerations

Gregory J. Buffone 1, John Savory 1, and J. Hermans 1

1 Depts. of Biochemistry, Pathology, Medicine, and Hospital Laboratories, University of North Carolina, Chapel Hill, N. C. 27514.

Development of centrifugal analyzers capable of making light-scattering measurements has prompted the investigation of the application of this type of instrumentation to the measurement of specific proteins. Investigation of antigen/antibody complex dimension and the kinetics of the IgG/anti-IgG reaction as followed by light scattering are examined in relation to improved analytical methodology. The effect of nonspecific protein on the reaction rate has been investigated for IgM, and data are presented that provide insight into the mechanism of the effect of periodic mixing.


Key Words: antigen/antibody reaction kinetics • effect of periodic mixing • apparent mol wt and radius of gyration as a function of presence of NaCl and polyethylene glycol • size of antigen/antibody complexes

Submitted on April 21, 1975
Accepted on August 11, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.