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Clinical Chemistry 21: 1766-1768, 1975;
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Clinical Chemistry, Vol 21, 1766-1768, Copyright © 1975 by the American Association for Clinical Chemistry

Enzymatic Immunoassay vs. Gas/Liquid Chromatography for Determination of Phenobarbital and Diphenylhydantoin in Serum

Harold E. Booker 1 and Barbara A. Darcey 1

1 The Epilepsy Center, Department of Neurology, University of Wisconsin Center for Health Sciences, Madison, Wis. 53706.

The antiepileptic drugs diphenylhydantoin and phenobarbital were measured in serum by a new commercially available enzyme immunoassay procedure ("EMIT," Syva Corp.). The procedure requires <5 min and no more than 50 µl of serum per determination. It is simple; only four steps (pipetting and diluting with an automatic pipettor-dilutor) are required before spectrophotometry. Twenty replicate analyses of a serum containing phenobarbital and diphenylhydantoin gave results with a CV of 6.8% and 9.1%, respectively. Results attained in a large series of patients were compared with results by a gas/liquid chromatographic procedure. For phenobarbital r = 0.97, and for diphenylhydantoin r = 0.98. No false negatives or false positives were encountered.

Submitted on May 6, 1975
Accepted on August 20, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.