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Clinical Chemistry 21: 1795-1798, 1975;
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Clinical Chemistry, Vol 21, 1795-1798, Copyright © 1975 by the American Association for Clinical Chemistry

Screening for Albuminuria: A Case for Estimation of Albumin in Urine

E. BenGershôm 1

1 Department of Clinical Chemistry, Academic Hospital Rotterdam/Sophia Children's Hospital and Neonatal Unit, Gordelweg 160, Rotterdam, The Netherlands.

The usefulness of bromcresol green for estimating albumin in urine was evaluated by comparison with the Laurell "rocket" technique. In contrast to the bromcresol green method applied for urinary albumin, rather doubtful results were obtained with conventional (Microzone) electrophoresis for albumin and with precipitation techniques for total protein estimation. Albumin estimation with bromcresol green is recommended as a more reliable substitute for total-protein estimations in urine. Limitations of bromcresol green are also pointed out.


Key Words: albumin vs. total-protein content of urine • routine urine analysis • bromcresol green for screening urines • intermethod comparison

Submitted on June 10, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.