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Clinical Chemistry 21: 1882-1886, 1975;
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Clinical Chemistry, Vol 21, 1882-1886, Copyright © 1975 by the American Association for Clinical Chemistry

Separation of Synthetic Mixtures of Sugars and of Sugars in Body Fluids with a High-Resolution Carbohydrate Analyzer

Wolfgang Voelter 1 and Hermann Bauer 1

1 Chemisches Institut, Universität Tübingen, 74 Tübingen, Germany.

With use of a specially designed gradient-generating system, of anion-exchange resins of 10-20 µm particle diameter, and of an orcinol solution in concentrated sulfuric acid (1 g/liter) as detection reagent, a mixture of 16 carbohydrates can be separated in less than 4 h. The limit of detection for most sugars is in the 0.1-2.0 nanomole range. The detection reagent is stable for months and does not become colored. The reagent mixes with the column effluent in an exothermic reaction and allows use of relatively short mixing coils. The analyzer is appropriate for investigating body fluids rapidly and with high sensitivity.


Key Words: carbohydrates in urine and cerebrospinal fluid • gradient-elution device

Submitted on June 27, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.