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Clinical Chemistry 21: 1903-1906, 1975;
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Clinical Chemistry, Vol 21, 1903-1906, Copyright © 1975 by the American Association for Clinical Chemistry

Immunofluorescence Method for Detecting Anti-Myocardial Antibodies, and Its Use in Diagnosing Heart Disease

Stanley L. Twomey 1 and George E. Bernett 1

1 Bio-Science Laboratories, Department of Research, 7600 Tyrone Ave., Van Nuys, Calif. 91405; and Good Samaritan Hospital, 1212 Shatto St., Los Angeles, Calif. 90017.

Demonstration of autoimmune antibodies to myocardial tissue enables one to detect and assess cardiac disease long after abnormalities in serum enzyme activities are no longer measurable. We describe an indirect immunofluorescence procedure in which cryostat sections of rat heart (ventricle) and Evan's Blue counterstaining are used to detect anti-myocardial antibodies. Sera from patients with myocardial infarct or some other cardiac diseases reveal a distinct fluorescent staining of the sarcolemmal membrane. In contrast, sera from patients with systemic lupus erythematosis demonstrate nuclear plus diffuse staining and sera from myasthenia gravis patients show a characteristic striated staining pattern. The role of anti-myocardial antibodies in cardiac disease is discussed briefly.


Key Words: systemic lupus erythematosis • • myocardial infarction • coronary insufficiency • diagnostic aid • myasthenia gravis

Submitted on June 25, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.