Clinical Chemistry
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Clinical Chemistry 21: 1927-1931, 1975;
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Clinical Chemistry, Vol 21, 1927-1931, Copyright © 1975 by the American Association for Clinical Chemistry

Relative Efficacy of Separation of "Free" and "Bound" [3',5'-3H]Pteroylglutamate by Charcoal Coated with Various Materials

Alfred Zettner 1 and Peggy E. Duly 1

1 Division of Clinical Pathology, Department of Pathology, School of Medicine, University of California, San Diego.

We studied the effectiveness with which various charcoal preparations separate free [3H]pteroylglutamate from that complexed with milk folate binder. We tested, in various concentrations, uncoated charcoals and charcoals coated with dextrans of various molecular weights, or with albumin, hemoglobin, or polyvinylpyrrolidone with an average molecular weight of 40 000. Although there was some distinction between the "bound" and "free" fractions with all charcoals, those treated with dextrans of average molecular weights of 43 500 or 70 000, or with polyvinylpyrrolidone gave the best separation over a greater range of charcoal concentration. Uncoated charcoal and charcoals coated with albumin, hemoglobin, or dextran T10 (average molecular weight, 10 500) were least effective.

Submitted on August 4, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.