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Clinical Chemistry 21: 1932-1934, 1975;
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Clinical Chemistry, Vol 21, 1932-1934, Copyright © 1975 by the American Association for Clinical Chemistry

Rapid Kinetic Measurement of Lactate in Plasma with a Centrifugal Analyzer

Michael A. Pesce 1, Selma H. Bodourian 1, and John F. Nicholson 1

1 Division of Clinical Chemistry, Babies Hospital, The Childrens Medical and Surgical Center of New York at Columbia Presbyterian Medical Center; and The Department of Pediatrics, College of Physicians and Surgeons of Columbia University, 3959 Broadway, New York, N. Y. 10032.

In this method, blood is collected in ammonium heparinized microhematocrit tubes and lactate is directly determined in the plasma, separated within 15 min from the erythrocytes. Lactate is assayed by mixing 10 µl of sample with NAD+ and lactate dehydrogenase in tris(hydroxymethyl)aminomethane hydrazine buffer. The rate of increase in absorbance of the NADH formed, measured at 340 nm, is proportional to lactate concentration. The assay is complete in 4 min and absorbance is linearly related to concentration from 0.625 to 15 mmol/ liter. Analytical recoveries of lactate added to plasma averaged 104% (range, 91-116%). Results compared well for plasma samples analyzed by this method with the CentrifiChem and the Du Pont aca.


Key Words: metabolic acidosis • diabetes • glycogen storage disease • hypoxemic shock • centrifugal analyzer • stability of lactate in plasma

Submitted on August 6, 1975




The following articles in journals at HighWire Press have cited this article:


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MYOCARDIAL SUBSTRATE UPTAKE AND OXIDATION DURING AND AFTER ROUTINECARDIAC SURGERY
J. Thorac. Cardiovasc. Surg., July 1, 1999; 118(1): 71 - 81.
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Copyright © 1975 by the American Association for Clinical Chemistry.