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Clinical Chemistry 21: 1953-1955, 1975;
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Clinical Chemistry, Vol 21, 1953-1955, Copyright © 1975 by the American Association for Clinical Chemistry

Identification of Human Hemoglobins by Use of Isoelectric Focusing in Gel

John A. Koepke 1, James F. Thoma 1, and Robert M. Schmidt 2

1 Laboratory Service, Veterans Administration Hospital, Iowa City, Iowa 52242.
2 Hematology Division, Center for Disease Control, Public Health Service, U. S. Department of Health, Education, and Wel fare, Atlanta, Ga. 30333.

Newly-developed gel isoelectric focusing techniques provide for rapid and correct identification of human hemoglobins. With this technique, we examined samples previously identified by the National Hemoglobinopathy Standardization Laboratory of the Center for Disease Control. A wide variety of both normal and abnormal hemoglobins was included in the 134 different specimens examined. The isoelectric points (pl's) were precisely determined; no discrepancies with results by standard techniques of identification were found. The method is accurate and precise. It is proposed as a powerful adjunct to other laboratory methods suitable for use in a hemoglobinopathy reference laboratory or specialized hematology service laboratory.






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Copyright © 1975 by the American Association for Clinical Chemistry.