Clinical Chemistry
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Clinical Chemistry 21: 186-189, 1975;
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Clinical Chemistry, Vol 21, 186-189, Copyright © 1975 by the American Association for Clinical Chemistry

Drug Screening by Enzymatic Immunoassay with the Centrifugal Analyzer

Alan Broughton 1 and Doris L. Ross 1

1 The University of Texas Medical School, Hermann Hospital, 1203 Ross Sterling Ave., Houston, Tex. 77025.

We describe a method for determination of barbiturates, amphetamines, opiates, and methadone by the "enzyme multiplied immunoassay technique" (EMIT) applied to the centrifugal analyzer. This method gives results that agree well (92% for barbiturates, 97% for opiates, 94% for methadone, and 96% for amphetamines) with those obtained by the same technique with the SyvaGilford EMIT instrumentation. Its advantages include use of half as much of the expensive reagents; capability of batch operation, which removes the need for continual attention; and more nearly complete automation. For individual samples it is as fast as the manual method; for large numbers of specimens it is faster.

Submitted on August 24, 1974
Accepted on November 5, 1974






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Copyright © 1975 by the American Association for Clinical Chemistry.