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Clinical Chemistry 21: 195-198, 1975;
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Clinical Chemistry, Vol 21, 195-198, Copyright © 1975 by the American Association for Clinical Chemistry

Immunochemical Determination of Serum Albumin with a Centrifugal Analyzer

Mogens Blom 1 and Niels Hjørne 1

1 Departments of Clinical Chemistry, Aalborg Hospital, 9000 Aalborg, Denmark.

The turbidity resulting from the reaction between albumin and specific anti-human serum was measured with a good precision by using a GEMSAEC centrifugal analyzer. The reaction was enhanced by polyethylene glycol to shorten the reaction time (5 min) and to displace the point of equivalence between antigen and antibody to an albumin concentration unlikely to occur in human sera (about 100 g/liter). An additional program for the computer was necessary to fit the absorbance readings of individual sera to the nonlinear standard curve. Serum albumin values obtained by the described method correlated well with values obtained by the electroimmuno-technique. About 100 samples could be analyzed per hour, 500 µl of 100-fold diluted antiserum being used per specimen.

Submitted on August 28, 1974
Accepted on November 5, 1974







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Copyright © 1975 by the American Association for Clinical Chemistry.