Clinical Chemistry
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Clinical Chemistry 21: 206-210, 1975;
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Clinical Chemistry, Vol 21, 206-210, Copyright © 1975 by the American Association for Clinical Chemistry

Routine Radioimmunoassay of Plasma Testosterone, and Results for Various Endocrine Disorders

Pui-Yuen Wong 1, Donald E. Wood 1, and Thomas Johnson 1

1 Toronto General Hospital, 101 College St., Toronto, Ontario, Canada M5G 1L7.

We describe a modification of published methods for radioimmunoassay of plasma testosterone. This simpler method involves no chromatographic steps, and the necessary reagents, including tritiated testosterone and testosterone anti-serum, are commercially available. A digital computer is used for the calculation. Without difficulty, a technician can complete 100 assays in three working days. Mean testosterone concentrations (± 2 SD) in the plasmas of 21 normal men and 26 women with a normal menstrual cycle were 684 ± 300 and 45 ± 2O ng/dl, respectively. Within- and between-assay precision (coefficient of variation) were 5.2% (n = 29) and 6.7% (n = 26), respectively. We have assayed more than 1000 samples during the past year. We give data on the concentrations of testosterone in plasma of patients with various endocrine disorders.


Key Words: diagnostic aid • endocrine disorders • normal values • steroids

Submitted on July 8, 1974
Accepted on November 6, 1974




The following articles in journals at HighWire Press have cited this article:


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 Eur J EndocrinolHome page
B. Auyeung, S. Baron-Cohen, E. Chapman, R. Knickmeyer, K. Taylor, and G. Hackett
Foetal testosterone and the child systemizing quotient
Eur. J. Endocrinol., November 1, 2006; 155(suppl_1): S123 - S130.
[Abstract] [Full Text] [PDF]


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Copyright © 1975 by the American Association for Clinical Chemistry.