Clinical Chemistry
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Clinical Chemistry 21: 227-229, 1975;
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Clinical Chemistry, Vol 21, 227-229, Copyright © 1975 by the American Association for Clinical Chemistry

Effects on Radioimmunoassay of Digoxin of Varying Incubation Periods for Antigen—Antibody Reaction and Varying Periods of Adsorption by Dextran-Coated Charcoal

Harumi Kuno-Sakai 1 and Hideto Sakai 1

1 Division of Experimental Immunology, Department of Medicine, University of Texas Medical Branch, Galveston, Tex. 77550.

In radioimmunoassay of digoxin by the dextran-coated charcoal method, duration of the incubation period for the antigen-antibody reaction and time allowed for dextran-coated charcoal to adsorb the free antigen are critical factors. We compared effects of prolonged periods of adsorption on results obtained with five commercial kits. In all cases, the digoxin concentrations increased linearly with adsorption time, but the increase varied among kits. Kallestad and New England Nuclear 3H-kits and the Squibb 125I-kit showed less increase (i.e., gave relatively consistent results after various periods of adsorption); the Schwarz/Mann 125I-kit and Clinical Assays 3H-kit showed greater increases. Precautionary steps are outlined for radioimmunoassays by this technique.


Key Words: "kit" methods

Submitted on August 15, 1973
Accepted on November 15, 1974






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Copyright © 1975 by the American Association for Clinical Chemistry.