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Clinical Chemistry 21: 260-263, 1975;
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Clinical Chemistry, Vol 21, 260-263, Copyright © 1975 by the American Association for Clinical Chemistry

Lecithin—Sphingomyelin Ratio in Amniotic Fluid, as Assessed by a Modified Thin-Layer Chromatographic Method in Which a Commercial Pre-Coated Plate Is Used

Valyacheri R. Mallikarjuneswara 1

1 Reproduction Laboratory, Department of Obstetrics & Gynecology, The Methodist Hospital, 506 Sixth St., Brooklyn, N. Y. 11215.

Commercially available pre-coated thin-layer chromatographic plates consisting of silica gel supported on aluminum sheets were used to determine the lecithinsphingomyelin ratio in samples of amniotic fluid. These lipids were separated in a chloroform-methanol-water (65:25:4 by vol) solvent system (lecithin, RF = 0.33; sphingomyelin, RF = 0.25), and the spots were made visible in 5-15 min by heating to 180 °C after spraying with a solution of copper acetate (30 g/liter) in aqueous phosphoric acid (80 g/liter). The lipids were measured densitometrically after "peeling off" the adsorbent containing the spots by using a transparent cellulose ("Scotch brand") adhesive tape. Virtues of the original Gluck procedure are retained without need to heat the plates to 280 °C after spraying with corrosive aqueous sulfuric acid (698 g/liter) to make the lipids visible. Resuits for synthetic mixtures in concentrations within the physiological range and with a ratio of 0.5 to 3.0 showed an accuracy of 99% ± 7% (SD).


Key Words: fetal lung maturity • respiratory distress syndrome • diagnostic aid • pediatric chemistry

Submitted on May 13, 1974
Accepted on November 25, 1974






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Copyright © 1975 by the American Association for Clinical Chemistry.