Clinical Chemistry
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Clinical Chemistry 21: 325-329, 1975;
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Clinical Chemistry, Vol 21, 325-329, Copyright © 1975 by the American Association for Clinical Chemistry

Assay of L-Tyrosine in Serum by Amperometric Measurement of Tyrosinase-catalyzed Oxygen Consumption

Anand Kumar 1 and Gary D. Christian 1

1 Department of Chemistry, University of Washington, Seattle, Wash. 98195.

A new enzymatic method for rapid direct measurement of serum tyrosine is described, based on the amperometric measurement of the rate of oxygen consumption when tyrosine is oxidized in the presence of the enzyme tyrosinase in a phosphate buffer (0.1 mol/liter, pH 7.4). A Beckman Glucose Analyzer with a Clark electrode is used for the measurement. The procedure does not require removal of serum protein, incubation nor extraction, and results are obtained in less than 1 mm. Results agree closely with those obtained by a fluorometric procedure and with an amino acid analyzer.


Key Words: phenylketonuria • thyroid disease • normal values • Beckman Glucose Analyzer

Accepted on December 2, 1974






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Copyright © 1975 by the American Association for Clinical Chemistry.