Clinical Chemistry
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Clinical Chemistry 21: 330-333, 1975;
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Clinical Chemistry, Vol 21, 330-333, Copyright © 1975 by the American Association for Clinical Chemistry

Effect of Lactate Dehydrogenase Isoenzymes on the Coupled Enzymatic Assay for Alanine Aminotransferase Activity

Michael M. Chang 1 and Tai Wha Chung 1

1 Division of Applied Biochemistry Laboratory, Korea Institute of Science and Technology, P. O. B. 131, Cheong-Ryang, Seoul, Korea.

We show an example of the importance of specifying the form of isoenzyme and source of indicator enzymes to be used in coupled enzymatic assays. When we compared H4 (pig heart) and M4 (rabbit muscle) isoenzymes of lactate dehydrogenase for their suitability as indicator enzymes in the assay for alanine aminotransferase activity, we found that about fourfold as much M4 as H4 was required in terms of lactate dehydrogenase activity to reflect accurately equivalent amounts of alanine aminotransferase activity. Moreover, the substrate specificities of the two isoenzymes differed quantitatively.

Submitted on June 17, 1974
Accepted on December 2, 1974






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Copyright © 1975 by the American Association for Clinical Chemistry.