Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 21: 353-355, 1975;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kesner, L.
Right arrow Articles by Chan, P. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kesner, L.
Right arrow Articles by Chan, P. C.

Clinical Chemistry, Vol 21, 353-355, Copyright © 1975 by the American Association for Clinical Chemistry

Determination of Orotic and Dihydroorotic Acids in Biological Fluids and Tissues

Leo Kesner 1, Frank L. Aronson 1, Morris Silverman 1, and Phillip C. Chan 1

1 Department of Biochemistry, Downstate Medical Center, State University of New York, 450 Clarkson Ave., Brooklyn, N. Y. 11203.

In commonly used procedures for colorimetric assay of orotic acid in biological materials, certain substances interfere, positively or negatively. Some that interfere negatively are ornithine, cysteine, citrulline, tyrosine, proline, hydroxyproline, and polyamines. Protein and interfering agents are easily removed by first isolating the total organic acid fraction. The orotic acid in this fraction can then be determined by an established colorimetric procedure. A rapid liquid—liquid column-chromatographic procedure is described, if analysis of both dihydroorotic and orotic acid is required.


Key Words: inherited disorders • interference by amino acids • column chromatography • colorimetry

Submitted on October 30, 1974
Accepted on December 6, 1974




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
A. B.P. van Kuilenburg, H. van Lenthe, M. Loffler, and A. H. van Gennip
Analysis of Pyrimidine Synthesis "de Novo" Intermediates in Urine and Dried Urine Filter- Paper Strips with HPLC-Electrospray Tandem Mass Spectrometry
Clin. Chem., November 1, 2004; 50(11): 2117 - 2124.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1975 by the American Association for Clinical Chemistry.