Clinical Chemistry
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Clinical Chemistry 21: 356-361, 1975;
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Clinical Chemistry, Vol 21, 356-361, Copyright © 1975 by the American Association for Clinical Chemistry

Interlaboratory Comparison of Enzymatic Methods for Serum Glucose Determination

Nathan Gochman 1, William T. Ryan 2, Rex E. Sterling 3, and Graham M. Widdowson 4

1 Laboratory Service, VA Hospital, San Diego, Calif. 92161 (to which address correspondence).
2 Beckman Instruments, Inc., Fullerton, Calif. 92634.
3 USC/ LAC Medical Center, Los Angeles, Calif. 90033.
4 Institute of Health Research, San Francisco, Calif. 94115.

This report describes an interlaboratory comparison of enzymatic serum glucose methods as currently applied in several instrumental adaptations. Four spectrophotometric, hexokinase-based methods used with the Du Pont "aca," the Abbott "Bichromatic Analyzer," the Aminco "Rotochem," and the Technicon "AutoAnalyzer II" were compared with glucose oxidase-based methods as used with the Beckman "Glucose Analyzer" and the "AutoAnalyzer II." We analyzed both normal samples and samples that contained potential interfering substances or were otherwise abnormal. Although all methods were satisfactorily precise, methodological bias was noted in several cases, particularly with abnormal specimens. The hexokinase-based methods were generally more variable. The most consistent data were obtained from the two glucose oxidase methods and the Du Pont aca. Results of this study comprises part of the background information required for development of an accurate glucose reference method under the auspices of the AACC Standards Committee.


Key Words: Du Pont aca, Abbott Bichromatic Analyzer, Aminco Rotochem, Technicon AutoAnalyzer, and Beckman Glucose Analyzer • glucose oxidase and hexokinase methods compared

Submitted on July 29, 1974
Accepted on December 9, 1974




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Copyright © 1975 by the American Association for Clinical Chemistry.