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Clinical Chemistry 21: 381-386, 1975;
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Clinical Chemistry, Vol 21, 381-386, Copyright © 1975 by the American Association for Clinical Chemistry

Electrophoresis on Cellulose Acetate and Chromatography on DEAE-Sephadex A-50 Compared in the Estimation of Creatine Kinase Isoenzymes

Walid G. Yasmineh 1 and Naomi Quast Hanson 1

1 Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Box 198, Mayo Memorial Building, Minneapolis, Minn. 55455.

We have compared two methods for separating the isoenzymes of creatine kinase in serum and measuring their activity: chromatography on diethylaminoethyl-. Sephadex A-50, with both continuous and discontinuous gradient elution, and electrophoresis on cellulose acetate. Results by continuous and discontinuous gradient elution correlated well both for tissue extracts and sera. Electrophoresis on cellulose acetate is evidently less sensitive than the chromatographic method for detecting and measuring low activities of the isoenzyme designated MB. We describe how the isoenzymes may be separated by discontinuous gradient elution from microcolumns of the Sephadex and their activity rapidly quantitated by the Rosalki method [J. Lab. Clin. Med. 69, 696 (1967)], with a centrifugal analyzer.


Key Words: diagnosis of myocardial infarction • heart disease • isoenzymes in extract of heart of human, pig, baboon, dog; human muscle; and dog brain • normal values • centrifugal analyzer

Submitted on November 12, 1974
Accepted on December 20, 1974




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