Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 21: 387-391, 1975;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jiang, N.-S.
Right arrow Articles by Wadel, O. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jiang, N.-S.
Right arrow Articles by Wadel, O. P.

Clinical Chemistry, Vol 21, 387-391, Copyright © 1975 by the American Association for Clinical Chemistry

Comparison of Clinical Assays for Serum Corticosteroids

Nai-Siang Jiang 1, Dwaine Machacek 1, and Orlando P. Wadel 1

1 Department of Laboratory Medicine, Mayo Clinic and Mayo Foundation, Rochester, Minn, 55901.

We compared results obtained by (a) chemical (spectrophotometric) and competitive protein-binding assays for serum corticosteroids, and (b) competitive proteinbinding and radioimmunoassay, with and without CCl4 extraction, for serum cortisol. The two corticosteroid methods gave identical results. Radioimmunoassay with and without a CCl4 extraction step gave identical results for cortisol. Competitive protein-binding assays for cortisol (with and without CCl4 extraction) and radioimmunoassay for cortisol gave similar results. Specimens with above-normal corticosterone or 11-deoxycortisol concentrations are an exception: only methods that included CCl4 extraction gave correct values for cortisol; methods without such an extraction step overestimated cortisol. The normal serum corticosteroid concentration (mean ± SD) was 175 ± 53 µg/liter for morning specimens, and 103 ± 40 µg/liter for specimens collected in the afternoon. For cortisol, the corresponding values were 162 ± 44 and 82 ± 29 µg/liter.


Key Words: inter-method comparison • normal values • radioimmunoassay • competitive binding assay • spectrophotometry • cortisol • 11-deoxycortisol • cross-reactivity of antiserum

Submitted on October 7, 1974
Accepted on December 20, 1974




The following articles in journals at HighWire Press have cited this article:


Home page
 Arch Gen PsychiatryHome page
J. C. Ritchie, B. J. Carroll, P. R. Olton, V. Shively, and M. Feinberg
Plasma Cortisol Determination for the Dexamethasone Suppression Test: Comparison of Competitive Protein-Binding and Commercial Radioimmunoassay Methods
Arch Gen Psychiatry, May 1, 1985; 42(5): 493 - 497.
[Abstract] [PDF]

Home page
 Arch Gen PsychiatryHome page
H. Y. Meltzer and V. S. Fang
Cortisol Determination and the Dexamethasone Suppression Test: A Review
Arch Gen Psychiatry, May 1, 1983; 40(5): 501 - 505.
[Abstract] [PDF]

Home page
 Arch Gen PsychiatryHome page
T. Maruta
Dexamethasone Suppression Test: Technique and Accuracy
Arch Gen Psychiatry, October 1, 1982; 39(10): 1217 - 1218.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1975 by the American Association for Clinical Chemistry.