Clinical Chemistry
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Clinical Chemistry 21: 392-397, 1975;
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Clinical Chemistry, Vol 21, 392-397, Copyright © 1975 by the American Association for Clinical Chemistry

Separation of Creatine Kinase Isoenzymes in Serum by Ion-Exchange Column Chromatography (Mercer's Method, Modified to Increase Sensitivity)

Daniel A. Nealon 1 and Arthur R. Henderson 1

1 Department of Clinical Biochemistry, University Hospital (University of Western Ontario), 339 Windermere Rd., London, Ontario N6G 2K3 Canada.

We describe our experience with Mercer's method [Clin. Chem. 20, 36 (1974)] for separating isoenzymes of creatine kinase (EC 2.7.3.2) in serum by ion-exchange chromatography. By using diethylaminoethyl cellulose rather than diethylaminoethyl Sephadex in the column and by changing the ionic strength of the eluting buffer, we could detect a significant proportion of the MB isoenzyme of serum creatine kinase at normal activities of creatine kinase in serum, even in the absence of cardiac disease.


Key Words: diagnosis of myocardial infarction • activity of isoenzyme MB in serum • heart disease • DEAE-cellulose and DEAE-Sephadex chromatography compared

Submitted on November 25, 1974
Accepted on December 23, 1974






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Copyright © 1975 by the American Association for Clinical Chemistry.