Clinical Chemistry
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Clinical Chemistry 21: 672-675, 1975;
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Clinical Chemistry, Vol 21, 672-675, Copyright © 1975 by the American Association for Clinical Chemistry

Comparison of Radioimmunoassay with Thin-Layer Chromatographic and Gas—Liquid Chromatographic Methods of Barbiturate Detection in Human Urine

David L. Roerig 1, Donna L. Lewand 1, Marilyn A. Mueller 1, and Richard I. H. Wang 1

1 Pharmacology Research Laboratory (151), Veterans Administration Center, Wood (Milwaukee), Wis. 53193; and the Department of Pharmacology, Medical College of Wisconsin, Milwaukee, Wis. 53233.

A radioimmunoassay (I) for barbiturates was compared with thin-layer chromatographic (II) and gas—liquid chromatographic (III) methods for barbiturate detection in human urine. Timed urine samples were obtained from volunteers who had ingested 100 mg of a barbiturate. I detected barbiturate in all urines tested up to 76 h after the dose, and III in all up to 52 h and in 90% up to 76 h. II detected barbiturates in 90% of all urine samples for only 30 h, after which its reliability declined. Glutethimide interfered with radioimmunoassay of barbiturate, producing false positives. I is sensitive, reliable, and fast, and lends itself to screening large numbers of urine samples for barbiturates. For routine urine surveillance, however, we found I to be less useful than II, which is still the method of choice. I has, however, proved to be an excellent method for confirming resuits of II.


Key Words: "kit" methods • screening • toxicology • inter-method comparison

Submitted on November 18, 1974
Accepted on February 3, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.