Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 21: 719-724, 1975;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, L. M. Y.
Right arrow Articles by Schiller, H. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, L. M. Y.
Right arrow Articles by Schiller, H. S.

Clinical Chemistry, Vol 21, 719-724, Copyright © 1975 by the American Association for Clinical Chemistry

Nonchromatographic Radioimmunoassay of Plasma 11-Deoxycortisol, for Use in the Metyrapone Test, with Polyethylene Glycol as the Precipitant

Lilian M. Y. Lee 1 and Harvey S. Schiller 1

1 Departments of Laboratory Medicine and Obstetrics and Gynecology, University of Washington School of Medicine, SB-10, Seattle, Wash. 98195.

We have developed a simple, reliable radioimmunoassay for plasma 11-deoxycortisol. The method does not require chromatography but instead makes use of a simple extraction procedure which, in combination with the antibody characteristics, is highly specific for the metyrapone test. Polyethylene glycol was used to separate free and antibody-bound steroid. The smallest amount measurable is 15 pg (2.0 µg/ liter of plasma). The method is shown to be precise and accurate. Intra-assay precision of the method for two plasma pools was 26.7 ± 2.5 µg/ liter (CV = 9.4%) and 61.2 ± 3.7 µg/ liter (CV = 6.0%). The respective inter-assay precision was 27.0 ± 1.7 µg/ liter (CV = 6.3%) and 59.9 ± 2.3 µg/ liter (CV = 3.8%). The validity of the assay was further verified by evaluating the plasma 11-deoxycortisol response to metyrapone administration. The relative simplicity of the method and the commercial availability of all reagents, including antisera, makes this radioimmunoassay procedure practical for use in clinical laboratories.


Key Words: evaluating adrenal and pituitary func tion

Submitted on November 12, 1974
Accepted on February 18, 1975






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1975 by the American Association for Clinical Chemistry.