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Clinical Chemistry 21: 730-734, 1975;
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Clinical Chemistry, Vol 21, 730-734, Copyright © 1975 by the American Association for Clinical Chemistry

Fluorometry of Citrate in Serum, with Use of Citrate (pro-3S)-Lyase

Arthur J. Tomisek 1, Edward M. Winkler , and Samuel Natelson 1

1 Department of Biochemistry, Michael Reese Hospital and Medical Center, Chicago, Ill. 60616.

We describe a procedure for enzymatic assay of citrate in human serum. The citrate is degraded to acetate and oxaloacetate with citrate oxaloacetate-lyase (pro-3S-CH2 · COO- rarr acetate) (EC 4.1.3.6). Some oxaloacetate loses CO2 to form pyruvate. Addition of malate and lactate dehydrogenases (EC 1.1.1.37 and 1.1.1.27) permits determination of the oxaloacetate and pyruvate generated, and thus of the citrate concentration. The decrease in NADH concentration is measured fluorometrically. Results obtained for 30 consecutive human sera by this procedure were compared to the procedure in which the citrate is converted to pentabromoacetone. There was no statistically significant difference in values obtained by the two procedures. The range of values (mean ± 2 SD) found for sera from 25 blood donors by this procedure was 12.8-27.2 mg/liter (mean, 19.0 mg/ liter). Serum citrate as measured by both procedures during a glucose tolerance test was decreased from initial values under the influence of administered glucose (and endogenous insulin). Insulin concentrations were also measured during these glucose-tolerance tests. Citrate concentrations remain subnormal after the glucose and insulin concentrations return to their initial values. This accords with published reports.


Key Words: intermethod comparison • normal values • glucose-tolerance test • convulsive seizures • insulin/glucose/citrate correlations

Submitted on January 9, 1975
Accepted on February 24, 1975




The following articles in journals at HighWire Press have cited this article:


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J. Lin, J. S. Hogan, and K. L. Smith
Antigenic Homology of the Inducible Ferric Citrate Receptor (FecA) of Coliform Bacteria Isolated from Herds with Naturally Occurring Bovine Intramammary Infections
Clin. Vaccine Immunol., November 1, 1999; 6(6): 966 - 969.
[Abstract] [Full Text] [PDF]


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Copyright © 1975 by the American Association for Clinical Chemistry.