Clinical Chemistry
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Clinical Chemistry 21: 751-754, 1975;
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Clinical Chemistry, Vol 21, 751-754, Copyright © 1975 by the American Association for Clinical Chemistry

Flash-Heater Ethylation of Some Antiepileptic Drugs

Patrick Friel 1 and Allan S. Troupin 1

1 Seizure Clinic, Department of Neurological Surgery, University of Washington School of Medicine, Seattle, Wash. 98195.

We describe a modification of the MacGee method [Anal. Chem. 42, 421 ( 1970)] for rapid determination of phenytoin (diphenylhydantoin) in plasma by gas—liquid chromatography, in which an ethylating reagent is used instead of the more common methylating reagents. With this modification, several N-methylated antiepileptic drugs can be separated from their demethylated metabolites. Our results agree well with those of Butler and Waddell [Neurology 8, 106 ( 1958)], who showed that in patients receiving the N-methylated compounds mephenytoin and mephobarbital, the N-demethylated products, 5-ethyl-5-phenylhydantoin and phenobarbital are found in higher concentrations in plasma than are their respective parent drugs. The method is also useful for routine determinations of phenobarbital, primidone, and phenytoin in serum, cerebrospinal fluid, or saliva.


Key Words: drugs in biological fluids • drug metabolite measurement • gas chromatography

Submitted on November 12, 1974
Accepted on February 5, 1975




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E. D. BURGESS, P. N. FRIEL, A. D. BLAIR Jr., and V. A. RAISYS
Serum Phenytoin Concentrations in Uremia
Ann Intern Med, January 1, 1981; 94(1): 59 - 60.
[Abstract] [PDF]


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Copyright © 1975 by the American Association for Clinical Chemistry.