Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 21: 754-757, 1975;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Clayson, K. J.
Right arrow Articles by Strandjord, P. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Clayson, K. J.
Right arrow Articles by Strandjord, P. E.

Clinical Chemistry, Vol 21, 754-757, Copyright © 1975 by the American Association for Clinical Chemistry

A More Sensitive Automated Method for Determination of Ornithine Carbamoyltransferase Activity in Human Serum

Kathleen J. Clayson 1, James S. Fine 1, and Paul E. Strandjord 1

1 Department of Laboratory Medicine, SB-10, University of Washington, Seattle, Wash. 98195.

Ornithine carbamoyltransferase (EC 2.1.3.3) activity is a sensitive, specific indicator of hepatocellular injury. This paper describes development of an improved automated procedure for measurement of this activity. Triethanolamine—ethylenedlaminetetraacetate is used as a buffer, and activity is determined by measuring the concentration of the product, citrulline. Kinetic studies have been performed to determine optimal pH and L-ornithine and carbamoyl phosphate concentrations. Recovery of citrulline was studied. The upper limit of normal obtained in a study of 106 blood-bank donors was 6 U/liter. The automated procedure developed as a result of these studies, in which optimal assay conditions are used, produces a threefold increase in sensitivity and permits use of a sample volume of 1 ml.


Key Words: continuous-flow method • liver disease • enzyme activity • normal values

Submitted on October 7, 1974
Accepted on February 3, 1975






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1975 by the American Association for Clinical Chemistry.