Clinical Chemistry
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Clinical Chemistry 21: 760-761, 1975;
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Clinical Chemistry, Vol 21, 760-761, Copyright © 1975 by the American Association for Clinical Chemistry

Preparation of a Modified Glucose Oxidase Reagent for the Polarographic Determination of Glucose with the Beckman "Glucose Analyzer"

Joseph Fischl 1, Dozcarona Federman 1, and Nira Talmor 1

1 The Department of Biochemistry, Beilinson Medical Center, Petah-Tikva, Israel.

In this modification, glucose oxidase, 140-180 U/ml, is dissolved in acetate buffer (pH 6):ethanol (96%):glycerol (80:10:10 by vol). Iodine and molybdate are used as catalysts, formaldehyde and mercuric iodide as preservatives, and octanol as an antifoaming agent. The reagent is suitable for use in the Beckman "Glucose Analyzer," it is stable at room temperature for at least six months, it is sensitive, it has a lubricating effect, and its cost is less than a fifth as much as the "Certified" Beckman reagent. It is not suitable for the measurement of glucose in samples containing maltose, cellobiose, gentiobiose, starch, and glycogen.

Submitted on November 22, 1974
Accepted on January 9, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.