Clinical Chemistry
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Clinical Chemistry 21: 765-768, 1975;
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Clinical Chemistry, Vol 21, 765-768, Copyright © 1975 by the American Association for Clinical Chemistry

Improved Estimation of Urinary Myoglobulin by Counterimmunoelectrophoresis, as Compared with the Double Immunodiffusion Technique

Hazem Hibrawi 1 and Robert G. Blaker 1

1 Bio-Science Laboratories, 7600 Tyrone Ave., Van Nuys, Calif. 91405.

We describe a simple, sensitive, and relatively inexpensive counterimmunoelectrophoretic technique for detection and estimation of myoglobin. The test can be completed within 90 min, compared with 12-48 h required by double immunodiffusion. With the counterimmunoelectrophoretic technique we could detect myoglobin in concentrations of 2-3 mg/liter, while the detection limit by double immunodiffusion was about 9 mg/liter.


Key Words: assessment of muscle damage, including myocardial infarction • emergency tests

Submitted on November 25, 1974
Accepted on February 13, 1975




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A. KOFFLER, R. M. FRIEDLER, and S. G. MASSRY
Acute Renal Failure Due to Nontraumatic Rhabdomyolysis
Ann Intern Med, July 1, 1976; 85(1): 23 - 28.
[Abstract] [PDF]


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Copyright © 1975 by the American Association for Clinical Chemistry.