Clinical Chemistry
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Clinical Chemistry 21: 768-770, 1975;
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Clinical Chemistry, Vol 21, 768-770, Copyright © 1975 by the American Association for Clinical Chemistry

Simple Manual Procedure for Determination of Serum Triglycerides

Jose Mendez 1, Barry Franklin 1, and Harry Gahagan 1

1 Laboratory for Human Performance Research, Intercollege Research Programs, 119 Noll Laboratory, The Pennsylvania State University, University Park, Pa. 16802.

We describe a modified method for determining serum triglycerides (triacylglycerols), which is based on the heptane extraction procedure of Gottfried and Rosenberg [Clin. Chem. 19, 1077 (1973)] with the stable saponification, oxidation, and color development reagents of Neri and Frings [Clin. Chem. 19, 1201 (1973)]. This modified method eliminates one heating step, reduces saponification time to 5 min, absorbances are read at room temperature, and the calibration curve is linear to 3.0 g/liter. A sample comparison between the proposed method and the automated Block and Jarrett [Am. J. Med. Technol. 35, 1 (1969)] procedure showed no significant difference (r = 0.98). The coefficient of variation (47 duplicate samples) for the modified method was 6.3%. Further validation was obtained from analysis of quality-control samples; the proposed method gave equivalent values.


Key Words: inter-method comparison • procedure for the small laboratory

Accepted on February 14, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.