Clinical Chemistry
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Clinical Chemistry 21: 770-773, 1975;
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Clinical Chemistry, Vol 21, 770-773, Copyright © 1975 by the American Association for Clinical Chemistry

Improved Radioimmunoassay of Plasma Cortisol

John Donohue 1 and Demetrios Sgoutas 1

1 Department of Pathology & Laboratory Medicine, Emory University School of Medicine, Atlanta, Ga. 30322.

We report a simplified radioimmunoassay procedure for determination of plasma cortisol. The method offers the advantage that tracer, antibody, and assay buffer are added in a single step with the use of semi-automated equipment. Thus, critical pipetting procedures are minimized, and assay time is reduced to 4 h. No prior extraction or chromatographic purification of cortisol is required. The procedure is simple, reliable, and accurate. For either between-assay or within-assay determinations, the coefficients of variation are about 6%. Our results compare well with those obtained from a procedure that included extraction and chromatographic purification of cortisol in several plasma samples. Conditions for optimizing the assay are also discussed.

Submitted on January 23, 1975
Accepted on February 24, 1975




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Copyright © 1975 by the American Association for Clinical Chemistry.