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Clinical Chemistry 21: 829-833, 1975;
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Clinical Chemistry, Vol 21, 829-833, Copyright © 1975 by the American Association for Clinical Chemistry

Computer-Controlled Automation of Radioimmunoassay Based on Gel Entrapment of Antibody

David B. Cottrell 1, E. Clifford Toren Jr. 1, Judith A. Magnuson 1, and Stuart J. Updike 1

1 Departments of Medicine and Pathology, University of Wisconsin, Madison, Wis. 53706.

We describe a new, broadly applicable approach to radioimmunoassay. Solid-phase binding reagent is prepared by entrapping antibody in polyacrylamide gel. This gel is then fragmented, sieved, and dehydrated in 95% ethanol. Upon evaporation of the ethanol, the dry antibody—gel particles are dispensed into miniature disposable plastic columns. A unidirectional flowing stream configuration is used to construct a standard curve for the polypeptide hormone, angiotensin I, used here to exemplify the technique. Good reproducibility of this standard curve is demonstrated. The analytical system includes a computer-controlled sample turntable, digital pipet, and liquid-switching assembly.


Key Words: angiotensin I • radioimmunoassay of low-molecular-weight haptens

Submitted on February 5, 1975
Accepted on March 12, 1975




The following articles in journals at HighWire Press have cited this article:


Home page
 ScienceHome page
G Brooker, W. Terasaki, and M. Price
Gammaflow: a completely automated radioimmunoassay system
Science, October 15, 1976; 194(4262): 270 - 276.
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Copyright © 1975 by the American Association for Clinical Chemistry.