Clinical Chemistry
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Clinical Chemistry 21: 834-837, 1975;
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Clinical Chemistry, Vol 21, 834-837, Copyright © 1975 by the American Association for Clinical Chemistry

Rapid Analysis for Theophylline in Serum by Use of High-Pressure Cation-Exchange Chromatography

Miles Weinberger 1 and Charles Chidsey 1

1 Division of Clinical Pharmacology (C237), University of Colorado Medical Center, 4200 E. Ninth Ave., Denver, Colo. 80220.

We have developed a quantitative assay for serum theophylline by high-pressure cation-exchange chromatography. As little as 0.1-ml aliquots of serum were prepared for analysis simply by diluting them with a solution of the internal standard (8-chlorotheophylline). Theophylline and the internal standard were eluted in 17 and 27.5 min, respectively, and the peaks for them were distinct from those of other xanthines and uric acids. We encountered no interference when we used: (a) plasma obtained from blood anticoagulated with citrate; (b) hemolyzed, lipemic, or icteric serum; or (c) 52 samples from patients who were receiving various other medications. An analysis of calibration suggested that errors greater than ±2.6 mg/liter, a clinically acceptable range, were highly unlikely. Sensitivity was sufficient to identify less than 2.5 mg/liter. This assay was compared with the usual method involving solvent extraction and absorbance measurement in the ultraviolet and found to have similar accuracy, although it is easier, faster, requires less sample, and potentially is more specific.


Key Words: toxicology • pediatric chemistry • spectrophotometry compared • monitoring therapy for asthma

Submitted on November 25, 1974
Accepted on March 17, 1975




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Copyright © 1975 by the American Association for Clinical Chemistry.