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Clinical Chemistry 21: 898, 1975;
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Clinical Chemistry, Vol 21, 898-898, Copyright © 1975 by the American Association for Clinical Chemistry

Inhibitory Effect of Fluoride on Glucose Tests with Glucose Oxidase Strips

John Onstad 1, Daniel Hancock 1, and Paul L. Wolf 1

1 University Medical Center, University of California—San Diego, La Jolla, Calif. 92037.

Sodium fluoride was inadvertently added as a preservative to the urine of an eight-year-old boy with diabetes mellitus before urinary glucose was measured. On preliminary screening of the urine, the test by glucose oxidase paper reagent strip gave a negative reading for glucose, whereas quantitative urinary glucose assay by the coupled enzyme reaction (hexokinase—glucose 6-phosphate dehydrogenase) gave a glucose concentration of 81.5 g/liter. Inadvertent use of sodium fluoride as a urine preservative may cause a falsely negative result with the glucose tests involving oxidase.

Submitted on February 17, 1975
Accepted on March 24, 1975







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Copyright © 1975 by the American Association for Clinical Chemistry.