Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 21: 1063-1066, 1975;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tsung, S. H.
Right arrow Articles by Milewski, K. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tsung, S. H.
Right arrow Articles by Milewski, K. A.

Clinical Chemistry, Vol 21, 1063-1066, Copyright © 1975 by the American Association for Clinical Chemistry

Immunological Measurement of Transferrin Compared with Chemical Measurement of Total Iron-Binding Capacity

Swei H. Tsung 1, Waldemar A. Rosenthal 1, and Karen A. Milewski 1

1 Clinical Chemistry Section, Laboratory Service/ 113, Veterans Administration Center, Wood (Milwaukee), Wis. 53193; and the Department of Pathology, The Medical College of Wisconsin, Milwaukee, Wis. 53226.

Because of uncertainty as to the molecular weight of transferrin, a previous comparison [Von der Heul et al., Clin. Chim. Acta 38, 347 (1972)] between transferrin content of serum and total iron-binding capacity cannot be definitive. We found a conversion factor for expressing transferrin as iron-binding capacity by measuring the maximum amount of iron bound by 1 mg of transferrin. We compared the resulting calculated value with values obtained by three other methods for measuring total iron-binding capacity. We agree with the previous observation that the latter, as measured radioisotopically, give higher results than would be judged from the transferrin content but the same as those for two chemical methods. The diffusion rate of transferrin in agar was the same irrespective of the degree of iron saturation. Serum transferrin concentrations were low in patients with anemia resulting from malignancy, chronic disorders, and cirrhosis of the liver, and high or normal in patients with iron deficiency anemia and in pregnant women or women who were taking birth-control pills. Measurement of transferrin concentration can be used to distinguish iron deficiency anemia from anemia resulting from chronic disorders, but offers no advantages over existing methods for estimating total ironbinding capacity.


Key Words: anemia • normal values • diagnostic aids • intermethod comparison • effect of estrogen on transferrin concentration • " kit" method • hepatic cirrhosis

Submitted on October 16, 1974
Accepted on April 7, 1975




The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
H. Yamanishi, S. Iyama, Y. Yamaguchi, Y. Kanakura, and Y. Iwatani
Modification of Fully Automated Total Iron-binding Capacity (TIBC) Assay in Serum and Comparison with Dimension TIBC Method
Clin. Chem., September 1, 2002; 48(9): 1565 - 1570.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
R. Gambino, E. Desvarieux, M. Orth, H. Matan, T. Ackattupathil, E. Lijoi, C. Wimmer, J. Bower, and E. Gunter
The relation between chemically measured total iron-binding capacity concentrations and immunologically measured transferrin concentrations in human serum
Clin. Chem., December 1, 1997; 43(12): 2408 - 2412.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1975 by the American Association for Clinical Chemistry.