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Clinical Chemistry 21: 1167-1168, 1975;
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Clinical Chemistry, Vol 21, 1167-1168, Copyright © 1975 by the American Association for Clinical Chemistry

Improved Colorimetric Determination of Salicylic Acid and Its Metabolites in Urine

Nagy A. Farid 1, Gordon S. Born 1, Wayne V. Kessler 1, S. M. Shaw 1, and W. E. Lange 1

1 Bionucleonics Dept., School of Pharmacy and Pharmacal Sciences, Purdue University, West Lafayette, Ind. 47907.

We describe an improvement in the Levy and Procknal method [J. Pharm. Sci. 57, 1330 (1968)] for determination of salicylic acid and its metabolites in urine. Salicylic acid and salicyluric acid are successively extracted from 1 or 2 ml of urine (acidified with HCl) by two 10-ml portions each of carbon tetrachloride and ethylene dichloride. The extracts of each solvent are shaken with 5 ml of ferric nitrate solution (a 10-fold dilution of 17 g of Fe(NO3)3·9H2O in 1 liter of 70 mmol/liter HNO3). The aqueous phases are centrifuged and their absorbances measured at 530 nm. For total salicylate, 3 ml of urine and 3 ml of HCl are heated in a partially evacuated serum vial at 100 °C for 16 h and then salicylic acid is assayed in the hydrolyzed sample. Recovery of a weighed oral dose of sodium salicylate in urine was 105.4%; it was 127.9% by the Levy and Procknal method for the same sample. The improved method is faster and more accurate.

Submitted on February 27, 1975
Accepted on April 10, 1975




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Copyright © 1975 by the American Association for Clinical Chemistry.