Clinical Chemistry
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Clinical Chemistry 21: 1175-1178, 1975;
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Clinical Chemistry, Vol 21, 1175-1178, Copyright © 1975 by the American Association for Clinical Chemistry

A Versatile Temperature-Controlled Reaction Cuvet

Michael G. Busby 1, David B. Cottrell 1, George S. Cembrowski 1, and E. Clifford Toren Jr. 1

1 University of Wisconsin Center for Health Sciences, Departments of Medicine and Pathology, Madison, Wis. 53706.

A thermostatted reaction cuvet, operating under computerized or manual temperature control in the ultraviolet or visible region, is described and evaluated. The cell was designed for use with the automated chemistry system described earlier [Clin. Chem. 19, 1114 (1973)], but can readily be adapted for other applications. The entire 3-ml cuvet assembly warms from ambient temperature to 37 °C in less than 10 min, with ±0.15 °C stability; the long-term stability is ±0.05 °C; temperature recovery time after washing or reagent addition is less than 3 min. The optical pathlength is 10.00 mm and the carryover volume is 18 µl.

Submitted on November 22, 1974
Accepted on April 30, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.