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Clinical Chemistry 21: 1217-1220, 1975;
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Clinical Chemistry, Vol 21, 1217-1220, Copyright © 1975 by the American Association for Clinical Chemistry

Automated Elution Electrophoresis: A Potential Clinical Tool

Charles D. Scott 1, Norman E. Lee 1, and Arthur W. Perkins 1

1 Oak Ridge National Laboratory,1 Oak Ridge, Tenn. 37830.

An elution electrophoresis system in which a porous packed bed is used for separation and a flow photometer or colorimeter for continuous monitoring of the eluate may be capable of rapid, high-resolution analysis of serum proteins and other protein mixtures with very little manual labor. In a prototype of such a system we used a cooled separations column, 3 mm in diameter and 40 cm long, containing polyacrylamide beads. Samples are introduced, via a microsyringe, through a septum at the column midpoint. Typical analyses for serum proteins or serum isoenzymes require an electrophoresis time of about 30 min at 1200 V.


Key Words: proteins • separation on column of polyacrylamide • isoenzyme and hemoglobin separation and assay • electrochromatography

Submitted on April 8, 1975
Accepted on May 19, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.