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Clinical Chemistry 21: 1234-1237, 1975;
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Clinical Chemistry, Vol 21, 1234-1237, Copyright © 1975 by the American Association for Clinical Chemistry

Application of Differential Light Scattering to the Latex Agglutination Assay for Rheumatoid Factor

Philip Blume 1 and Leonard J. Greenberg 1

1 Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minn. 55455.

When a suspension of particles is irradiated with a collimated beam of monochromatic polarized light of a wavelength close to the particle size and the intensity of the scattered light is measured as a function of angle, the scatter intensity is characterized by a series of relative maxima and minima. The nature of the signal depends on several significant variables that are characteristic of the particles. We have constructed a differential light scatter photometer and have applied the technique to analysis of rheumatoid factor by using latex particles coated with fraction II gamma-globulin. The results suggest that such a photometer may have potential applications in antigen-antibody assays based on the use of sensitized particles.


Key Words: laser • antigen-antibody reactions

Submitted on March 28, 1975
Accepted on May 12, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.