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Clinical Chemistry 21: 1258-1262, 1975;
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Clinical Chemistry, Vol 21, 1258-1262, Copyright © 1975 by the American Association for Clinical Chemistry

High-Speed Quantitative Karyotyping by Flow Microfluorometry

J. W. Gray 1, A. V. Carrano 1, D. H. Moore II 1, L. L. Steinmetz 1, J. Minkler 1, B. H. Mayall 1, M. L. Mendelsohn 1, and M. A. Van Dilla 1

1 Biomedical Division, Lawrence Livermore Laboratory, University of California, Livermore, Calif. 94550.

We describe a new flow-system technique for highspeed quantitative karyotyping. Metaphase chromosomes are isolated from cells, stained with a DNA-specific fluorochrome, and individually measured for stain content at rates up to 105/min in a flow microfluorometer. The resulting distribution contains quantitative information about the relative frequency of occurrence and the chromosomal DNA content of the measured chromosomes. Such analyses of Chinese hamster chromosomes are in good agreement with independent results from scanning cytophotometry and with cytogenetic observations of the banded chromosomes. Analyses of two karyotypicaliy different clones of Chinese hamster M3-1 lines provide the quantitative characterization of two stable translocations. Initial data on human chromosomes are presented and agree well with results of scanning cytophotometry and suggest potential clinical applications as preparative and instrumental techniques improve.


Key Words: assessing translocations • DNAspecific fluorochrome • DNA distribution in the chromosome as a reflection of karyotype • cytogenetics • intermethod comparison

Submitted on April 24, 1975
Accepted on May 29, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.