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Clinical Chemistry 21: 1323-1328, 1975;
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Clinical Chemistry, Vol 21, 1323-1328, Copyright © 1975 by the American Association for Clinical Chemistry

Application of Electron Spin Resonance to Determination of Serum Drug Concentrations

Mark R. Montgomery 1, Jordan L. Holtzman 1, and Richard K. Leute 1

1 Clinical Pharmacology Section, Veterans Administration Hospital, Minneapolis, Minn. 55417; Departments of Pharmacology and Medicine, University of Minnesota, Minneapolis, Minn. 55455; and Syva Research Institute, Palo Alto, Calif. 94304.

Displacement of spin-labeled drugs from drug-specific antibodies in human serum can be measured by use of electron spin resonance. This "spin immunoassay" has advantages of speed, simplicity, and small volume requirements over other standard techniques such as gas—liquid, liquid—liquid, or thin-layer chromatography. Unlike radioimmunoassay, there is no need to separate free and bound label and there are no radiation hazards. We present clinical and experimental data showing how this method can be applied to the routine determination of morphine and diphenylhydantoin concentrations in serum.


Key Words: spin-labeling vs. radiolabeling • diphenylhydantoin • morphine • inter-method comparison • toxicology

Submitted on March 20, 1975
Accepted on May 24, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.