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Clinical Chemistry, Vol 22, 32-38, Copyright © 1976 by American Association for Clinical Chemistry
D Apter, O Janne, P Karvonen and R Vihko
We describe a method for determination of pregnenolone, progesterone, 17alpha-hydroxyprogesterone, testosterone, and 5alpha- dihydrotestosterone in 1-2 ml of serum from male or female. Using microcolumns of Lipidex-5000 (hydroxyalkoxypropyl Sephadex, 0.5 g) and light petroleum/chloroform (97/3) as the solvent during chromatography, we resolved these five steroids into four fractions, with pregnenolone and 5alpha-dihydrotestosterone eluting together. By use of selected antibodies, the latter two steroids were also determined specifically. Use of microcolumns allowed minimization of solvent volumes and sample transfers. Consequently, blank values for all the five steroids were negligible. Lowest measureable concentrations (in ng/liter) were: pregnenolone 100, progesterone 25, 17alpha-hydroxyprogesterone 50, testosterone 25, and 5alpha-dihydrotestosterone 25. Intra-assay and inter-assay coefficients of variation ranged from 5 to 9% and 10 to 15%, respectively, for the five steroids. Serum concentrations of these steroids are given for women in the follicular and luteal phases of the menstrual cycle and for women on oral contraceptives of the combination type, as well as for normal men.
The following articles in journals at HighWire Press have cited this article:
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  M. Hero, O. A. Janne, K. Nanto-Salonen, L. Dunkel, and T. Raivio Circulating Antiandrogenic Activity in Children with Congenital Adrenal Hyperplasia during Peroral Flutamide Treatment J. Clin. Endocrinol. Metab., September 1, 2005; 90(9): 5141 - 5145. [Abstract] [Full Text] [PDF]
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A. M. Wikstrom, T. Raivio, F. Hadziselimovic, S. Wikstrom, T. Tuuri, and L. Dunkel Klinefelter Syndrome in Adolescence: Onset of Puberty Is Associated with Accelerated Germ Cell Depletion J. Clin. Endocrinol. Metab., May 1, 2004; 89(5): 2263 - 2270. [Abstract] [Full Text] [PDF]
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T. Raivio, L. Dunkel, S. Wickman, and O. A. Janne Serum Androgen Bioactivity in Adolescence: A Longitudinal Study of Boys with Constitutional Delay of Puberty J. Clin. Endocrinol. Metab., March 1, 2004; 89(3): 1188 - 1192. [Abstract] [Full Text] [PDF]
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S. Wickman, E. Kajantie, and L. Dunkel Effects of Suppression of Estrogen Action by the P450 Aromatase Inhibitor Letrozole on Bone Mineral Density and Bone Turnover in Pubertal Boys J. Clin. Endocrinol. Metab., August 1, 2003; 88(8): 3785 - 3793. [Abstract] [Full Text] [PDF]
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 T. Raivio, J. S. Tapanainen, P. Kunelius, and O. A. Janne Serum Androgen Bioactivity During 5{alpha}-Dihydrotestosterone Treatment in Elderly Men J Androl, November 1, 2002; 23(6): 919 - 921. [Abstract] [Full Text] [PDF]
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S. Wickman and L. Dunkel Inhibition of P450 Aromatase Enhances Gonadotropin Secretion in Early and Midpubertal Boys: Evidence for a Pituitary Site of Action of Endogenous E J. Clin. Endocrinol. Metab., October 1, 2001; 86(10): 4887 - 4894. [Abstract] [Full Text] [PDF]
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T. Raivio, J. J. Palvimo, L. Dunkel, S. Wickman, and O. A. Jänne Novel Assay for Determination of Androgen Bioactivity in Human Serum J. Clin. Endocrinol. Metab., March 1, 2001; 86(4): 1539 - 1544. [Abstract] [Full Text]
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 T. Sarkola, T. Fukunaga, H. Makisalo, and C. J. Peter Eriksson ACUTE EFFECT OF ALCOHOL ON ANDROGENS IN PREMENOPAUSAL WOMEN Alcohol Alcohol., January 1, 2000; 35(1): 84 - 90. [Abstract] [Full Text] [PDF]
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 M. Tena-Sempere, A. Rannikko, J. Kero, F.-P. Zhang, and I. T. Huhtaniemi Molecular Mechanisms of Reappearance of Luteinizing Hormone Receptor Expression and Function in Rat Testis after Selective Leydig Cell Destruction by Ethylene Dimethane Sulfonate Endocrinology, August 1, 1997; 138(8): 3340 - 3348. [Abstract] [Full Text] [PDF]
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