Clinical Chemistry
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Clinical Chemistry 22: 67-69, 1976;
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Clinical Chemistry, Vol 22, 67-69, Copyright © 1976 by American Association for Clinical Chemistry

Improved microscale assay for purine phosphoribosyltransferase activities

R Schmidt, M Foret and U Reichert

We describe an improved and rapid filter-type assay for purine phosphoribosyltransferase activities. 14C-labeled purine bases are used as substrates, and the equipment includes a sampling manifold and glass- fiber filters coated with polyethyleneimine-cellulose. The method is especially suited for assay of a large number of samples, and may be useful for other enzyme activity measurements.




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Copyright © 1976 by the American Association for Clinical Chemistry.