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Clinical Chemistry 22: 92-97, 1976;
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Clinical Chemistry, Vol 22, 92-97, Copyright © 1976 by American Association for Clinical Chemistry

Improved separation of creatine kinase cardiac isoenzyme in serum by batch fractionation

LG Morin

I describe a simple, single-tube batch fractionation procedure for separating MM and MB isoenzymes of creatine kinase on a macroporous strong anion exchanger (AG MP-1, Bio-Rad Laboratories). The isoenzymes can be separated in less than 3 min, with a resulting dilution of the serum with no more than an equal volume of buffer. Without sample concentration or spectrofluorometric measurement, the procedure detects 4 U of MB isoenzyme per liter. Sensitivity is limited by the sensitivity and precision of the method of measurement. The CV for the fractionation can be held to less than 4.0% at 65 U of MB per liter. Current fractionation methods are compared to the proposed procedure. With use of a discrete analyzer (Du Pont aca) the mean MB activity in a population free of heart disease was 3.2 +/- 3.0 U/liter (range, 0 to 8 U/liter). The kinetics and stability of isolated isoenzymes are reported, indicating that advisability of storing or pre-incubating samples with mercaptoethanol.


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Home page
 Mol. Pathol.Home page
N Durany, J Carreras, M Valenti, J Camara, and J Carreras
Inactivation of phosphoglycerate mutase and creatine kinase isoenzymes in human serum
Mol. Pathol., August 1, 2002; 55(4): 242 - 249.
[Abstract] [Full Text] [PDF]


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Copyright © 1976 by the American Association for Clinical Chemistry.