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Clinical Chemistry 22: 1586-1591, 1976;
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Clinical Chemistry, Vol 22, 1586-1591, Copyright © 1976 by American Association for Clinical Chemistry

Microcalorimetric determination of glucose in reference samples of serum

RN Goldberg

Ireport a detailed series of microcalorimetric measurement of glucose concentrations in five reference samples of serum. The method utilized (a) calibration in the actual medium of analysis and (b) a correction for interferences owing to the nonspecificity of the enzyme hexokinase. The microcalorimetric results are compared with the tentative results of analyses obtained by the spectrophotometric hexokinase/glucose-6- phosphate dehydrogenase method and by isotope-dilution mass spectrometry. In most cases, the microcalorimetric results appear to be in agreement with the results obtained by these latter two methods. A discussion of the basis of the microcalorimetric measurements is presented.




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Copyright © 1976 by the American Association for Clinical Chemistry.